Queue: The queue leads guests indoors through very simple hallways set up like a 2D tutorial level with various interactive blocks that once touched will trigger corresponding sounds and simple projected animations. After the tutorial queue guests will load into their Yoshi egg "doom buggie" style carts.

Ride: The ride itself will take guests through various "world levels" typically found in Mario games featuring the Mushroom Kingdom, a snow level, a fire level, a desert level, and an ocean level housed in different rooms with simple Mario animatronics as well as various enemies from classic Mario games. The ride will then enter the boss fight against Bowser who will breathe "fire" (the room will be heated much like Hell in Mr Toads Wild Ride) before Mario saves the day. As you exit onto your moving platform Toad tells you to come back again for Your Princess is in another castle.